Application of coenzyme self-sufficient chassis cell in biosynthesis

• Main Authors: ZHENG YUGUO, TANG HENG, XUE YAPING, ZHAO JINQIAO
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an application of a coenzyme self-sufficient chassis cell in biosynthesis. The coenzyme self-sufficient chassis cell carries an NAD kinase with an amino acid sequence as shown in SEQ ID NO.1 and a recombinant expression vector of a coding gene of a mutant of the NAD kinase. The NAD kinase mutant is obtained by carrying out single-point mutation or multi-point superposition mutation on the 110th site, the 170th site and the 259th site of an amino acid sequence as shown in SEQ ID NO.1. The coenzyme self-sufficient chassis cell can be used for biosynthesis of a pranlukast intermediate 3AHAP, ursodesoxycholic acid UDCA and a remegapam intermediate compound II, the coenzyme self-sufficient chassis cell is introduced into a multi-enzyme system to convert NAD < + > into NADP < + >, the NADPH is reduced by glucose dehydrogenase GDH to generate coenzyme NADPH, and the coenzyme NADPH can be used for biosynthesis of a pranlukast intermediate 3AHAP, ursodesoxycholic acid UDCA and a remegapam inter