SgRNA for accurately editing bovine Oct4 and application of sgRNA

The invention relates to the technical field of gene engineering and cell engineering, and discloses sgRNA for precisely editing bovine Oct4 and application of the sgRNA, the sgRNA for precisely editing bovine Oct4 comprises the sgRNA for precisely editing bovine Oct4, the nucleotide sequence of the...

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Main Authors ZHONG JIFENG, CAO SHAOXIAN, ZHAO FANG, DING QIANG, XIA SHUWEN, WANG HUILI, ZHANG YUGE, CHEN KUNLIN
Format Patent
LanguageChinese
English
Published 01.03.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to the technical field of gene engineering and cell engineering, and discloses sgRNA for precisely editing bovine Oct4 and application of the sgRNA, the sgRNA for precisely editing bovine Oct4 comprises the sgRNA for precisely editing bovine Oct4, the nucleotide sequence of the sgRNA is shown as SEQ ID NO: 1, the sgRNA further comprises a primer for amplifying the sgRNA, and the primer is used for amplifying the bovine Oct4. The primers comprise a 840 site primer, an Oct4 in vitro cutting template amplification primer and a B840-px330 in vitro transcription template amplification primer. The sgRNA is used for accurately editing the bovine Oct4 gene, the sgRNA is designed for a fourth exon region of the bovine Oct4 gene, and upstream and downstream specific homologous arms capable of realizing efficient homologous recombination are designed for cleavage sites; a Crispr-Cas9 technology is utilized, a targeted endogenous Oct4 specific site is accurately cut, and GFP (Green Fluorescent Prote
Bibliography:Application Number: CN202311679228