Expression of biomolecules with improved promoters and TIR

• Main Authors: MUNDHADA HEMANT, NELSON ALEX T, CHANDRASEKARAN, PURUSHOTTAM
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure relates to a genetically modified host cell that produces serine that expresses or overexpresses an operon comprising one or more genes encoding: a 3-phosphopyruvate dehydrogenase (serA) that converts D-3-phosphopyruvate (PGA) to phosphohydroxypyruvate (PHP); a phosphoserine transaminase (serC) for converting the 3-phosphohydroxypyruvic acid into phosphoserine, and a phosphoserine transaminase (serC) for the phosphoserine; and/or a phosphoserine phosphatase (serB) that converts phosphoserine to L-serine wherein the operon is operably linked to a constitutive promoter having a set of nucleotide sequences that are at least 95% (e.g., at least 96%, e.g., at least 97%, e.g., at least 98%, e.g., at least 99%, e.g., 100%) identical to the constitutive promoter comprised in SEQ ID NO: 8. 本公开涉及产生丝氨酸的经基因修饰的宿主细胞，其表达或过表达操纵子，所述操纵子包含编码以下的一个或多个基因：将D-3-磷酸丙酮酸(PGA)转化为磷酸羟基丙酮酸(PHP)的3-磷酸丙酮酸脱氢酶(serA)；将3-磷酸羟基丙酮酸转化为磷酸丝氨酸的磷酸丝氨酸转氨酶(serC)；和/或将磷酸丝氨酸转化为L-丝氨酸的磷酸丝氨酸磷酸酶(serB)，其中操纵子可操作地连接至具有与SEQ ID NO:8中包含的组成型启动子至少95％