Anti-human CD20 antibody or antigen binding fragment thereof as well as preparation method and application thereof

• Main Authors: CHU BING'ER, ZHANG YA, TANG XIAOBIN, CHU JIANDA, XU FEI, SHI JIAMIN, ZHANG YANG, ZHAO XINPING
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the technical field of biology, and provides an anti-human CD20 antibody or an antigen binding fragment thereof, a light chain variable region of the antibody has an amino acid sequence shown as SEQ ID NO: 1-3, or has at least one amino acid difference compared with the amino acid sequence shown as SEQ ID NO: 1-3; the heavy chain variable region has an amino acid sequence as shown in SEQ ID NO: 4-6, or has at least one amino acid difference compared with the amino acid sequence as shown in SEQ ID NO: 4-6. The mouse anti-human CD20 antibody provided by the invention has affinity close to that of a commercial anti-human CD20 antibody, has good specificity, and can specifically recognize a closed CD20 target antigen. 本发明属于生物技术领域，提供了一种抗人CD20抗体或其抗原结合片段，其轻链可变区具有如SEQ ID NO:1-3所示的氨基酸序列，或与其相比具有至少1个氨基酸差异；重链可变区具有如SEQ ID NO:4-6所示的氨基酸序列，或与其相比具有至少1个氨基酸差异。本发明提供的鼠抗人CD20抗体与商品化抗人CD20抗体亲和力接近，特异性良好，可特异性识别封闭CD20靶抗原。