Method for detecting concentration of anti-human lymphocyte immune globulin active antibody

• Main Authors: XU KUANHONG, ZOU HAOYONG, GENG PENG, WANG YINLIN, YANG FAN, YU ZEQIONG, YIN WENQU, YANG JINLIANG, NIE XILIN, YU QINYU, ZHOU XIAOLU
• Format: Patent
• Language: Chinese; English
• Published: 27.02.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for detecting the concentration of an active antibody in anti-human lymphocyte immune globulin. The concentration of the active antibody in the anti-human lymphocyte immune globulin is detected by combining enzyme-linked immunosorbent assay (ELISA) and flow cytometry. The method comprises the following steps: firstly, adsorbing a to-be-detected sample by using immune cells, then detecting the reduced proportion of active antibodies in the to-be-detected sample before and after incubation and adsorption by using flow cytometry, and then detecting the reduced concentration of total antibodies in the to-be-detected sample before and after incubation and adsorption by virtue of an ELISA (Enzyme-Linked Immuno Sorbent Assay) experiment. And calculating to obtain the concentration of the active antibody in the to-be-detected sample and the proportion of the active antibody in the total IgG antibody. The method can be used for detecting the concentration of active antibodies in raw mat