Anti-pIgR nano antibody and application thereof

• Main Authors: WU JIANGBO, DING CHAOYANG
• Format: Patent
• Language: Chinese; English
• Published: 27.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an anti-pIgR nano antibody and application thereof, the nano antibody is PI-19, PI-21, PI-28, PI-5 and PI-74, and the amino acid sequences of the nano antibody are respectively as shown in SEQ ID NO: 10, SEQ ID NO: 3, SEQ ID NO: 2, SEQ ID NO: 13 and SEQ ID NO: 27. The nano antibody capable of being combined with pIgR in a high affinity manner is obtained by screening through a phage display library. The anti-pIgR nano antibody obtained by screening is combined with the pIgR with high affinity, can be used for preparing medicines for treating pIgR related diseases, and provides a new means for treating the diseases. 本发明公开了一种抗pIgR纳米抗体及其应用，该纳米抗体为PI-19、PI-21、PI-28、PI-5、PI-74，其氨基酸序列分别如SEQ ID NO：10、SEQ ID NO：3、SEQ ID NO：2、SEQ ID NO：13和SEQ ID NO：27所示。本发明利用噬菌体展示文库筛选得到能够与pIgR高亲和性结合的纳米抗体。本发明筛选得到的抗pIgR的纳米抗体高亲和性结合pIgR，可用于制备治疗pIgR相关疾病的药物，为治疗这类疾病提供了新手段。