Recombinant streptococcus protein G, affinity chromatography resin and application thereof

• Main Authors: XIONG YUHANG, ZHAO YIKUN, FU KANG, HU JUN'AN
• Format: Patent
• Language: Chinese; English
• Published: 27.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SEPARATION; SPIRITS; TESTING; TRANSPORTING; VINEGAR; WINE

The invention discloses a recombinant streptococcus protein G, affinity chromatography resin and application thereof, and relates to the technical field of recombinant proteins. The amino acid sequence of the streptococcus protein G is optimized and truncated through a large number of screening, it is found that the recombinant streptococcus protein G with the amino acid sequence shown in SEQ ID NO.1 is easy to express in plasmids, by means of glycosylation modification of pichia pastoris, the expression quantity of the recombinant streptococcus protein G can be increased, and stable expression can be achieved. The streptococcus protein G provided by the invention can be specifically bound with IgG, and has higher binding force compared with the existing Protein G. The streptococcus protein G can be coupled with agarose, is used for preparing affinity chromatography resin, and is used for separating and purifying biomolecules such as antibodies. A marker is coupled to the streptococcus protein G, and the stre