DNA (deoxyribonucleic acid) nanoprobe with intelligent form reconstruction core-shell structure as well as preparation method and application of DNA nanoprobe

• Main Authors: SHEN ZHIFA, YANG YUANBING, WU ZAISHENG, PAN WENHAO, LIU JICHENG, CHEN LINHUAN
• Format: Patent
• Language: Chinese; English
• Published: 23.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to the technical field of miRNA (micro Ribonucleic Acid) detection, in particular to a DNA (Deoxyribose Nucleic Acid) nanoprobe with an intelligent morphological reconstruction core-shell structure as well as a preparation method and application of the DNA nanoprobe. A shell DNA tetrahedron of the DNA nanoprobe provided by the invention is connected with a middle layer DNA triplex through a disulfide bond modified DNA sequence, and glutathione (GSH) can induce reduction stimulation response; the deoxyribozyme part of the DNA triplex is designed as miRNA-21 complementary binding activation, the catalytic activity center of the deoxyribozyme chain is recovered, a substrate is cut, and a detectable fluorescence signal is released; a cholesterol core is taken as an inner layer hydrophobic part, when the DNA nanoprobe is in contact with a cell membrane, the DNA nanoprobe is subjected to intelligent form reconstruction, the hydrophobic part is in the outer layer, the hydrophilic part is in the