Cationic hybrid nano-protein entrapped with biodegradable enzyme as well as preparation and application of cationic hybrid nano-protein

• Main Authors: ZHAO PENGFEI, LIU DELIANG, ZHENG MINGBIN, ZHOU YANG, HE ZHUOJUN, LU HONGZHOU, DAI GUIQIN
• Format: Patent
• Language: Chinese; English
• Published: 20.02.2024
• Subjects: CHEMISTRY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention discloses a cationic hybrid nano-protein entrapped with a biodegradable enzyme as well as preparation and application of the cationic hybrid nano-protein. The method comprises the following steps: dissolving lactoferrin and a reducing agent in water together, and reacting to obtain a reduced lactoferrin solution; mixing the reduced lactoferrin solution with the biodegradable enzyme solution, then adjusting the pH value to 6-8 to obtain a mixed solution, then adding absolute ethyl alcohol, and mixing and homogenizing to obtain a mixed solution; and carrying out ultrafiltration concentration on the mixed solution. The cationic hybrid nano-protein disclosed by the invention targets a biofilm part with negative electricity through an electrostatic adsorption effect and releases cellulase to disintegrate a bacterial biofilm; the self-contained cation can be adsorbed and combined with a lipopolysaccharide layer with negative electricity in a bacterial wall to induce the selective permeability loss of