Method for detecting target nucleic acid and nucleic acid sequence of adjacent region of target nucleic acid

• Main Authors: ZHU XIUHUANG, LIU JIANHONG, DENG TAO, SUN LICHAO, WANG JIAO, CHANG YUJUN
• Format: Patent
• Language: Chinese; English
• Published: 13.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biology, and particularly relates to a method for detecting a target nucleic acid and a nucleotide sequence of an adjacent region thereof. Specifically, CRISPR-Cas9 targets the middle position of a target gene sequence, the target gene sequence and the sequence information on the two sides of the target gene are obtained at the same time by connecting a nanopore sequencing joint and extending from the middle of the target gene to the two sides of the target gene, effective detection of the target gene is improved, and meanwhile the advantages of long reading length sequencing detection can be utilized; sequence information of nucleic acid adjacent to the physical position of the target gene is directly obtained, and mining of important functional information such as species annotation and upstream and downstream sequence analysis of the target gene is achieved. 本发明属于生物技术领域，具体涉及一种检测目标核酸及其邻近区域核酸序列的方法。具体地，本发明通过CRISPR-Cas9靶向靶基因序列的中间位置，通过连接纳米孔测序接头，从靶基因的中间向两侧延伸，同时获取靶基