Method for producing intestinal nerve precursor cells

• Main Authors: YAMASHITA TERUYOSHI, IKEYA MAKOTO, TAKE KAZUMI, KAMIYA DAISUKE, KAMIYA YAYOI
• Format: Patent
• Language: Chinese; English
• Published: 13.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided is a method for producing enteric nerve precursor cells, which is a technique for culturing and proliferating enteric nerve precursor cells while maintaining the ability to differentiate into enteric nerve cells and glial cells, said method comprising: a step (1) for providing enteric nerve precursor cells; and a step (2) for culturing the intestinal nerve precursor cells in a culture medium containing an ERBB3 agonist and/or an ERBB4 agonist. 作为一种用于使肠神经前体细胞保持分化为肠神经细胞和胶质细胞的分化能力的同时对其进行培养并使其增殖的技术，提供一种肠神经前体细胞的制造方法，该方法包括：工序(1)，提供肠神经前体细胞；以及工序(2)，在包含ERBB3激动剂和/或ERBB4激动剂的培养基中培养肠神经前体细胞。