Fritillaria cirrhosa tissue culture method capable of preventing browning and application of fritillaria cirrhosa tissue culture method

• Main Authors: HE SHUQI, YUAN YUAN, HAN GUANGMING, ZHANG XIAOYU, LIANG YANLI, HE SIMEI, PENG SHI, ZHANG GUOWU, ZHANG XIAODONG, MENG ZHENGUI, CHEN YONGSHENG, ZHAO YAN, HAN JUN, WU LIANG, LU YINGCHUN, XU YANJIAO
• Format: Patent
• Language: Chinese; English
• Published: 06.02.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a bulbus fritillariae cirrhosae tissue culture method capable of preventing browning, which comprises the following steps: taking fresh bulbus fritillariae cirrhosae bulbs as explants, and carrying out cleaning and disinfection, pretreatment before inoculation and pre-culture; a culture medium used in the pre-culture comprises the following components: 25 to 35 g/L of MS (Murashige and Skoog) and cane sugar, 6 to 7.6 g/L of agar, 0.5 to 1.5 mg/L of IBA (Indolebutyric Acid), 0.5 to 1.5 mg/L of NAA (Naphthalene Acetic Acid), 0.3 to 0.7 mg/L of 2, 4-D and 0.1 The bulbus fritillariae cirrhosae tissue culture method has the advantages that bulbus fritillariae cirrhosae medicinal material seedlings obtained by adopting the tissue culture method are good in growth vigor, free of browning, uniform in color, good in overall appearance and relatively high in product quality. 本发明公开了一种能防止褐化的川贝母组织培养方法，包括以下步骤：取川贝母新鲜鳞茎作为外植体，进行清洗消毒、接种前预处理和预培养；所述预培养使用的培养基包括如下组分：MS，蔗糖25～35g/L，琼脂6～7.6g/L，IBA0.5～1.5mg/L，N