Method for obtaining hevea brasiliensis homozygous gene editing plant

• Main Authors: LIN QIUFEI, YANG XIANFENG, UDAYABANU, GENU, HUANG TIANDAI, DENG YUTING, GU XIAOCHUAN
• Format: Patent
• Language: Chinese; English
• Published: 02.02.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a method for obtaining a hevea brasiliensis homozygous gene editing plant, and relates to the technical field of gene engineering. The method comprises the following steps: carrying out enzyme digestion on a gene editing vector to obtain an enzyme digestion vector, connecting the enzyme digestion vector with a target sequence to obtain a connection product, transforming the connection product into escherichia coli to neutralize agrobacterium to obtain transformed bacteria, infecting a hevea brasiliensis somatic embryo, and the like to obtain a hevea brasiliensis homozygous gene editing plant. According to the invention, a gene editing vector is used as a transformation donor, whether positive resistant somatic embryos are chimeras is judged through high-throughput sequencing of the positive resistant somatic embryos, and T0-generation resistant somatic embryos are all chimeras. According to the method, the obtained T0-generation positive chimera embryo is cut and purified and then is co