Method for selectively extracting DHA (docosahexaenoic acid) from microalgae based on bis-imidazole ionic liquid

• Main Authors: WU YUE, ZHU LILI, LI BANGXU, WEN LILI, SONG ZONGWEI
• Format: Patent
• Language: Chinese; English
• Published: 02.02.2024
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY

The invention discloses a method for selectively extracting DHA (docosahexaenoic acid) from microalgae based on biimidazole ionic liquid, which comprises the following steps of: adding an alcoholic solution of alkali into microalgae grease serving as a raw material, and heating and refluxing; then adding deionized water and a bis-imidazole ionic liquid, and standing for layering; and taking the lower-layer solution, adding acid to adjust the pH value, extracting with an organic solvent, drying, and concentrating under reduced pressure to obtain the DHA algal oil finished product. The adopted bis-imidazole ionic liquid can selectively extract DHA in microalgae, the extraction rate and purity are high, the selectivity is good, and the method has a very good industrial production application prospect. 本发明公开了一种基于双咪唑类离子液体选择性从微藻中提取DHA的方法，以微藻油脂为原料，加入碱的醇溶液，加热回流；然后加入去离子水和双咪唑类离子液体，静置分层；取下层溶液，加入酸调节pH，经有机溶剂萃取、干燥、减压浓缩得DHA藻油成品。本发明采用的双咪唑类离子液体可以对微藻中的DHA进行选择性提取，提取率和纯度高，选择性好，具有很好的工业生产应用前景。