Method for efficiently doubling corn haploid

• Main Authors: ZHANG LIANG, CHEN CHUANYONG, FU XIUYI, ZHANG XUEYUAN, WU SHANSHAN, CHEN CHEN, ZHAO JIURAN, ZHANG HUASHENG, DUAN MINXIAO, GUO CHENG'EN, PENG YANKE, ZHANG CHUNYUAN, WANG YUANDONG
• Format: Patent
• Language: Chinese; English
• Published: 30.01.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; TRAPPING

The invention discloses a method for efficiently doubling corn haploids, and belongs to the field of breeding. In order to solve the technical problem of how to solve the problem of low DH production efficiency when haploid immature embryo doubling is carried out by colchicine, the invention provides a composition which comprises glycine, vitamin B1, nicotinic acid and vitamin B6. In the composition, the ratio of glycine to vitamin B1 to nicotinic acid to vitamin B6 is 20: 1: 5: 5. The invention further provides application of the composition in maize haploid immature embryo doubling, a feasible scheme is provided for improving the haploid immature embryo tissue culture identification and doubling efficiency under the effect of colchicine and achieving efficient haploid doubling, and the efficiency of a haploid breeding technology can be greatly improved. 本发明公开了一种玉米单倍体高效加倍的方法，属于育种领域。本发明要解决的技术问题是如何解决秋水仙素进行单倍体幼胚加倍时存在DH生产效率低的问题，为解决上述技术问题本发明提供一种组合物，所述组合物包括甘氨酸、维生素B1、烟酸和维生素B6。所述组合物中，甘氨酸：维生素B1：烟酸：维生素B6的比值为20：1：5：5。本