Methods of enhancing efficacy of isolated cell therapy for cell therapy

• Main Authors: KALINSKI, HAGAR, ZUR DANIEL, FEINSTEIN ELENA
• Format: Patent
• Language: Chinese; English
• Published: 26.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present disclosure relates to a method of enhancing the therapeutic efficacy of an isolated cell for use in cell therapy, such as adoptive cell transfer therapy, by inserting a low-expression miRNA that is beneficial for the therapeutic efficacy of cell therapy into an actively expressed locus of a gene that is protein-encoding or non-encoding, it obstructs the therapeutic efficacy of cell therapy by this disruption of the expression of the latter while inducing the expression of the former. 本公开涉及增强分离的细胞的治疗功效的方法，该分离的细胞用于细胞疗法，诸如过继性细胞转移疗法，该方法通过将对细胞疗法的治疗功效有益的低表达的miRNA插入基因的活跃表达的基因座中，该基因是蛋白质编码或非编码的，其通过这种破坏后者的表达同时诱导前者的表达而妨碍细胞疗法的治疗功效。