CRBN gene humanized animal model, construction system, construction method and application

• Main Authors: ZHU WEIBIN, YUE HANGQI, HAN ZHICHENG, CHEN ZHONGHENG, ZHONG JIANGHONG
• Format: Patent
• Language: Chinese; English
• Published: 23.01.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; NEW BREEDS OF ANIMALS; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TRAPPING

The invention relates to the technical field of animal gene engineering, in particular to a CRBN gene humanized animal model, a construction system, a construction method and application, C57BL/6J strain mice are adopted, and C57BL/6J is called B6 for short; specifically, a breeding cage is established by adopting mice of a B6. Fastlpr/lpr strain, a B6. Ncf1m1j/m1j strain, a B6. Crbntm1.1 Ble strain, a B6. Stat1fl/fl strain, a B6. Cd68Cre/Cre strain and a B6. Lyz2Akaluc strain, and cross breeding is performed to finally obtain animal models of the four kinds of mice; according to the macrophage tracing method combined with Ncf1 gene polymorphism and Lyz2 gene targeted autofluorescence labeling, a Cre labeled mouse is transcribed through the promoter position of a membrane protein Cd68 coding gene, and after the Cre labeled mouse and a Stat1-Floxp mouse are copulated, a conditional gene knockout CKO mouse is established, and a multi-control mouse model with a wide application range is established; and immune d