Liquid chromatography-mass spectrometry detection method for multiple antituberculosis drugs in serum

• Main Authors: ZHANG RUOYING, ZHOU FENG, GUO SUHANG, XU YINGYING, LI JINMENG, CAI XINJUN, FAN XUDONG
• Format: Patent
• Language: Chinese; English
• Published: 19.01.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a liquid chromatography-mass spectrometry detection method for multiple anti-tuberculosis drugs in serum. The method comprises the following steps: (1) establishing a standard curve; (2) sample pretreatment; and (3) detecting the content of the sample by adopting an ultra-high performance liquid chromatography-mass spectrometer. The liquid chromatography-mass spectrometry detection method disclosed by the invention can be used for detecting 10 common anti-multidrug-resistant tuberculosis drugs in human serum at one time, the serum dosage in the analysis process is small, the pretreatment method is simple, the cost is low, the analysis is rapid, the selectivity, the residual effect, the matrix effect, the accuracy and the precision meet the requirements, and the liquid chromatography-mass spectrometry detection method can be used for quantitative analysis of the serum anti-tuberculosis drugs. 本发明涉及一种血清中多种抗结核病药物的液质联用检测方法，该方法包括以下步骤：(1)建立标准曲线；(2)样品预处理；(3)采用超高效液相色谱-质谱联用仪对样品含量进行检测。本发明的液质联用检