Tissue culture method of ornamental aquatic plant Danish red rose

• Main Authors: LIU HAIYING, HAN JINGLING, DUAN NAN, WANG NAIHONG, HUANG DONGHAI
• Format: Patent
• Language: Chinese; English
• Published: 19.01.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a tissue culture method of ornamental aquatic plant Danish red rose. The tissue culture method comprises the following steps: step 1, preparing an explant; sterile cups for production are selected, seedlings grow robustly and are sterile, and the plant height is 1/2-2/3 of the cup height; and 2, differentiation. After the culture medium, roots and miscellaneous leaves are removed, stems containing 1-2 leaf buds are cut and transferred to a differential culture medium, and differentiated seedlings are obtained; and step 3, proliferation. Transferring the differentiated seedlings to a proliferation culture medium to obtain proliferation seedlings; 4, rooting. Transferring the proliferated seedling single plant to a rooting culture medium to obtain a tissue culture seedling, repeating the step 2 to the step 4 to continuously obtain the tissue culture seedling, or directly culturing aquatic plants. The obtained tissue culture seedlings are high in multiplication coefficient, free of plant d