UCH-L1 protein quantitative detection kit and application

• Main Authors: ZHANG XIAOLI, WU FEIFEI, ZHONG FANGHU, HUANG YUE, YU YUAN, CHEN DAWEI, WANG YUAN, ZHOU YAN, FAN FENGYAN, LIN KAI
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a UCH-L1 protein quantitative detection kit and application, and belongs to the technical field of medical examination. The kit provided by the invention comprises a nitrocellulose film, a polyester pad and a sample pad, the nitrocellulose film comprises a UCH-L1 protein antibody for detection and a quality control antibody; the polyester pad comprises a fluorescent microsphere labeled antibody conjugate; the sample pad is soaked in a sample pad buffer solution, the sample pad buffer solution comprises the following components: 1L of the sample pad buffer solution contains 12.0 to 15.0 g of tris (hydroxymethyl) aminomethane, 3.0 to 12 g of S9, 0.2 to 2.0 g of cholic acid, 0.2 to 5 g of casein, 5 to 30 g of PVP-40 and the balance of water, and the pH value of the sample pad buffer solution is 7.8 to 9.0. The kit disclosed by the invention is simple and convenient to operate, short in reaction time, relatively high in sensitivity and suitable for on-site rapid detection, and has a very go