Probe, kit and drop-off ddPCR method for quantitatively detecting IDH1 gene mutation

• Main Authors: SHANG YUHONG, SHU YINGSHUANG, WANG BING, LI XUAN, YANG CHUNYAN, ZHOU QIMING
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of gene detection, and particularly relates to a probe, a kit and a drop-off ddPCR method for quantitatively detecting IDH1 gene mutation. Two probes with different fluorescence signals are designed according to an IDH1 gene exon 4 hot spot mutation region, a Drop-off probe is located on a mutation hot spot region, and the probes are complementary with a wild type sequence but not complementary with a mutation site; the Reference probe is located outside a mutation hot spot area, and the probe is complementary with a mutant type sequence and a wild type sequence. As long as a Drop-off probe coverage area is mutated, the probe cannot be combined with a template sequence, at the moment, a Reference probe can still be combined with the Drop-off probe coverage area to amplify, finally, a mutant type, a wild type and a negative type are distinguished according to the fluorescence signal intensity of liquid drops measured by a detection instrument and the position of the