Additive for PCR (Polymerase Chain Reaction) amplification, multiplex PCR amplification kit and application of multiplex PCR amplification kit

• Main Authors: KANG HUANHUAN, BI JING, GAO YIFAN, ZHANG GUOLIANG, ZHOU LETIAN, JU MINGXIA, ZENG LIDONG
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an additive for PCR (Polymerase Chain Reaction) amplification, a multiplex PCR amplification kit and application thereof. The additive contains benzalkonium bromide. After the additive is added into a single PCR system or a multiple PCR system, the additive has a relatively great promotion effect on amplification efficiency and amplification specificity of single PCR amplification and multiple PCR amplification, and has an important application prospect in single PCR or multiple PCR. 本发明提供了用于PCR扩增的添加剂、多重PCR扩增试剂盒及其应用，所述添加剂中含有苯扎溴铵。本发明所述添加剂添加在单重PCR体系或多重PCR体系后，对单重PCR扩增和多重PCR扩增的扩增效率和扩增特异性均有较大的促进的作用，在单重PCR或多重PCR中具有重要的应用前景。