Method for rapidly screening specific SNP (Single Nucleotide Polymorphism) probe based on quantum dot platform

• Main Authors: FANG MEIHONG, QIU HUILIANG, ZHOU NA, HUANG SINAN, CHEN SIYI, WANG HAILIN, ZHU HAITAO
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a method for rapidly screening specific SNP (Single Nucleotide Polymorphism) probes based on a quantum dot platform, which comprises the following steps: firstly, designing and synthesizing two forward NH2 modified probes (wild type and mutant type) and two reverse biotin modified oligonucleotides (wild type and mutant type); then the two probes are hybridized with wild oligonucleotides and mutant oligonucleotides respectively based on a quantum dot nucleic acid detection method, the specificity of the probes is determined, and the specific SNP probes can be obtained through rapid screening. The method disclosed by the invention is simple in design, the conventional synthesized long double-stranded DNA is used as a positive control, and the short single-stranded oligonucleotide only containing the SNP site is used as the positive control, so that the synthesis period and the synthesis cost are reduced; meanwhile, the steps of PCR amplification, denaturation before nucleic acid hybridiza