Method for separating bacteroides and degrading Fuzhuan tea polysaccharide by using bacteroides

• Main Authors: SHI JIAMENG, ZENG XIAOXIONG
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The invention relates to a method for separating bacteroides and degrading Fuzhuan tea polysaccharide by using bacteroides. The Fuzhuan tea polysaccharide is separated and purified from Fuzhuan tea in Anhua of Hunan, the neutral sugar content of the Fuzhuan tea polysaccharide is 48.63%, the uronic acid content of the Fuzhuan tea polysaccharide is 52.14%, and the Fuzhuan tea polysaccharide is heteropolysaccharide with the molecular weight of 20.5 kDa and composed of galacturonic acid, galactose, arabinose, rhamnose, glucose and mannose according to the molar ratio of 55.2: 16.5: 10.2: 8.9: 6.6: 2.5. After the Fuzhuan tea polysaccharide is ingested by a human body, the Fuzhuan tea polysaccharide is not degraded in the upper digestive tract but is degraded and utilized by intestinal microorganisms in the colon, the bacteroid is a main degradation person of complex glycans, and the experiment aims at obtaining the bacteroid strain capable of degrading the Fuzhuan tea polysaccharide through separation and purifica