Response surface methodology-optimized extraction method and application of crude polysaccharide of Xinjiang large-fruit elaeagnus angustifolia

• Main Authors: TANG HUI, JIANG ZHENGTAO, WANG ZHENQI, LIAO BINGWU, LIU YINGFEI, SUN YAN, GAO SHANG
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2024
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention discloses a green source-saving response surface method optimized Xinjiang elaeagnus angustifolia crude polysaccharide (EMPc) extraction process. The process comprises the following steps: crushing and sieving elaeagnus angustifolia, degreasing soluble impurities, performing hot water reflux extraction, performing concentration and alcohol precipitation, performing centrifugation, and performing freeze drying; according to the method, an ethanol degreasing-hot water extraction method is adopted for extraction, the extraction process of the crude polysaccharide of elaeagnus angustifolia is optimized through combination of a single factor and a response surface test, parameters are optimized through a response surface method, and the optimization conditions are as follows: when the extraction temperature is 92 DEG C, the extraction time is 5 hours and the liquid-material ratio is 1: 37 (g/mL), the extraction rate of EMPc is about 3.88%. The efficient and green extraction of the crude polysaccharid