Method for detecting content of water-soluble vitamins in milk powder

• Main Authors: LIANG JINGJING, CHEN BILIAN, YUE CHAO, LIU ZHU, ZHU BINAN, DING YUQI, CHEN WANQIN, ZHOU XIA
• Format: Patent
• Language: Chinese; English
• Published: 12.01.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting the content of water-soluble vitamins in milk powder, which is characterized in that a three-column two-dimensional gel chromatography-tandem mass spectrometry technology is applied to rapid, efficient and sensitive detection of the water-soluble vitamins in the milk powder for the first time by preparing a sample solution, a linear solution, a blank solution and a standard recovery test sample solution. According to the method, pretreatment is simple, online purification is achieved, gel column online purification liquid chromatography-tandem mass spectrometry is successfully applied to multi-component vitamin analysis, and an online purification liquid chromatography-tandem mass spectrometry method is established for determining the content of various water-soluble vitamins in milk powder. A brand-new rapid detection method and an analysis process which is simple and easy to operate are established, human intervention factors are reduced, the recovery rate and