Living cell aerosol inhalation device and use method

• Main Authors: ZANG WEIZHONG, ARP ACAL
• Format: Patent
• Language: Chinese; English
• Published: 12.01.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROMTHE BODY; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a living cell aerosol inhalation device and a use method thereof. The use method comprises the following steps: S1, carrying out in-vitro culture on living cells needing aerosol inhalation; s2, carrying out related quality inspection on the living cells cultured in vitro, and identifying specific living cell markers; s3, centrifuging the living cells cultured in vitro to remove supernate, then adding a certain amount of living cell culture fluid and lung cell protection fluid, and resuspending; s4, cryopreserving the living cells; s5, resuscitating the living cells; and S6, preparing a cell atomizing agent, and continuously atomizing through an atomizing device. According to the application, through in-vitro cell culture, the problem of cell viability after cell atomization can be improved by improving the in-vitro cell culture medium; the atomization inhalation device is arranged and has the advantage of being portable, cells are inhaled into the lung after being atomized, and the prob