Self-purified nucleic acid-encoded libraries

• Main Authors: KELLER MICHAEL, KELLMANN, JU RG, NERI DARIO, BASSI GABRIELE, PETROV DIMITAR, KODATSU YUICHI
• Format: Patent
• Language: Chinese; English
• Published: 09.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to methods for producing nucleic acid-encoded compounds and libraries of nucleic acid-encoded compounds. A neogenic compound comprising a scaffold attached to a solid support by a linker is covalently attached to one or more chemical structural units to form a chemical moiety attached to the scaffold. A coding oligonucleotide encoding the one or more chemical structural units is covalently linked to the neogenic compound to form a coding nucleic acid moiety linked to the scaffold. A cleavage group is linked to the chemical moiety, nucleic acid moiety or scaffold of the compound. The linker is then reacted with the cleavage group such that the linker is cleaved and the compound is released from the solid support. Nucleic acid-encoded compounds and libraries and methods for their production are provided. 本发明涉及用于产生核酸编码的化合物和核酸编码的化合物的文库的方法。包含通过接头连接至固体载体的支架的新生化合物共价连接至一个或多个化学结构单元以形成连接至所述支架的化学部分。将编码所述一个或多个化学结构单元的编码寡核苷酸共价连接至所述新生化合物以形成连接至所述支架的编码核酸部分。将切割基团连接至所述化合物的所述化学部分、核酸部分或支架。然后使所述接头与所述切