Derf1 recombinant antigen as well as preparation method and application thereof

• Main Authors: MENG DEZHI, LIU WANJIAN, SHAN JINHONG, YANG FAN, WANG TING, SUN YUKE, ZHANG YUANYUAN, YANG ZHITING
• Format: Patent
• Language: Chinese; English
• Published: 09.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to the technical field of biology, in particular to a Derf1 recombinant antigen and a preparation method and application thereof, the Derf1 recombinant antigen is a recombinant antigen containing a Derf1 protein fragment I, a Derf1 protein fragment II and a Derf1 protein fragment III, the Derf1 protein fragment I is 200 amino acids from the 99th site to the 298th site in Derf1 protein, the Derf1 protein fragment II is 14 amino acids from the 99th site to the 128th site in the Derf1 protein, and the Derf1 protein fragment III is a Derf1 protein fragment III. And the Derf1 protein fragment III is 24 amino acids from the 244th site to the 267th site in the Derf1 protein. The Derf1 recombinant antigen is used in a dermatophagoides farinae allergy IgE antibody detection kit, the detection sensitivity is greatly improved, and the false positive rate is reduced. 本发明涉及生物技术领域，尤其涉及一种Derf1重组抗原、其制备方法及应用，Derf1重组抗原为包含Derf1蛋白片段I、Derf1蛋白片段II以及Derf1蛋白片段III的重组抗原，所述Derf1蛋白片段I为Derf1蛋白中第99位至第298位的200个氨基酸，所述D