Recombinant adeno-associated virus for expressing ENO1 human-mouse chimeric antibody and preparation method of recombinant adeno-associated virus

• Main Authors: DAI PENGYU, ZHANG TINGTING, MA XINYUN, CHO WON-WOOK, ZHU BINGDONG, LIU HUILING, YANG RUI, ZHOU JUANHONG, PEI YAPING, YANG NAN
• Format: Patent
• Language: Chinese; English
• Published: 05.01.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention is applicable to the technical field of biological medicines, and provides a preparation method of a recombinant adeno-associated virus for expressing an ENO1 human-mouse chimeric antibody, which comprises the following steps: constructing and identifying rAAV-ENO1-ximab plasmid; and preparing the rAAV-ENO1-ximab recombinant adeno-associated virus. The invention also provides the rAAV-ENO1-ximab recombinant adeno-associated virus prepared by the preparation method of the recombinant adeno-associated virus for expressing the ENO1 human-mouse chimeric antibody, and the rAAV-ENO1-ximab recombinant adeno-associated virus is obtained by the preparation method of the recombinant adeno-associated virus. The invention also provides an application of the rAAV-ENO1-ximab recombinant adeno-associated virus in preparation of drugs for inhibiting proliferation, migration or glycolysis of cervical cancer cells. The rAAV-ENO1-ximmAb recombinant adeno-associated virus prepared by the invention has an obvious in