Method for identifying rice oil doped in soybean oil based on oleic acid stable carbon isotope ratio

• Main Authors: LEI CHUNNI, ZHANG HUI, WANG YUE, GU QIANG, YI XIAOJUAN, WU WEIYAN
• Format: Patent
• Language: Chinese; English
• Published: 22.12.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for identifying rice oil doped in soybean oil based on oleic acid stable carbon isotope ratio, which comprises the following steps: 1, taking a vegetable oil sample to be detected, adding isooctane to fully dissolve, uniformly stirring, adding a KOH-methanol solution to react, and standing until the solution is clear after the reaction is finished; adding sodium hydrogen sulfate to neutralize the residual KOH, standing, taking supernate, adding isooctane, diluting and uniformly mixing to obtain a solution to be detected; 2, measuring the delta 13C value of methyl oleate in the solution to be measured, wherein instrument conditions comprise that a gas chromatography-stable isotope mass spectrometer is adopted for measurement; 3, calculating; 4, comparing the calculated delta 13CFA C18: 1 numerical value of the oleic acid (C18: 1) of the to-be-detected solution with a judgment threshold value, and if the delta 13CFA C18: 1 numerical value is greater than or equal to the judgment