Digital micro-droplet RNA amplification detection system for prostatic cancer and application of PCA3 gene in system

• Main Authors: JU JINLIANG, ZHANG SHIQI, CUI ZHENLING, RAN PENGZHAN
• Format: Patent
• Language: Chinese; English
• Published: 22.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a digital micro-droplet RNA amplification detection system for prostatic cancer and application of a PCA3 gene in the system, and belongs to the technical field of tumor diagnosis. The digital micro-droplet RNA amplification detection system for prostatic cancer comprises reagents for respectively and specifically detecting the following genes based on a real-time fluorescent nucleic acid isothermal amplification method: PCA3 and SPDEF, and a digital PCR system. Compared with the existing kit product, the digital micro-droplet RNA amplification detection system provided by the invention has the advantage that the detection cost can be greatly reduced on the basis of ensuring higher detection sensitivity and accuracy. 本发明公开一种前列腺癌的数字微液滴RNA扩增检测系统及PCA3基因在该系统中的应用，属于肿瘤诊断技术领域。提供的前列腺癌的数字微液滴RNA扩增检测系统包括基于实时荧光核酸恒温扩增方法的分别特异性检测以下基因的试剂：PCA3和SPDEF，和数字PCR系统。本发明提供的数字微液滴RNA扩增检测系统相比于现有试剂盒产品可以在保证较高的检测灵敏度和准确度的基础上大大降低检测成本。