Construction method and application of human-derived multiple myeloma in-situ tumorigenesis mouse model with high osteolytic damage

• Main Authors: LIU PENG, WANG YUE, LAN TIANWEI
• Format: Patent
• Language: Chinese; English
• Published: 22.12.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a construction method and application of a human-derived multiple myeloma in-situ tumorigenesis mouse model with high osteolytic damage. According to the invention, a human multiple myeloma cell line RPMI-8226 is subjected to gene modification, and a human multiple myeloma orthotopic transplantation tumor model of mouse multiple osteolytic bone injury is established. The technical problem that a multiple myeloma human-derived multiple myeloma in-situ tumorigenesis model capable of keeping bone marrow microenvironment integrity is lacked at present is solved. Besides, after the CXCL7 is over-expressed, the biological behavior of an RPMI-8226 cell line is transformed, so that multiple osteolytic bone damage of mice can be obviously caused, the physiological and pathological processes and osteolytic damage of human multiple myeloma can be well simulated, and a high-quality transplantation tumor model is provided for exploring the pathophysiological mechanism of multiple myeloma and screen