Anti-human PD-1 mouse monoclonal antibody as well as preparation method and application thereof

• Main Authors: CHU BING'ER, ZHANG YA, TANG XIAOBIN, CHU JIANDA, XU FEI, SHI JIAMIN, ZHANG YANG, ZHAO XINPING
• Format: Patent
• Language: Chinese; English
• Published: 22.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides an anti-human PD-1 mouse monoclonal antibody as well as a preparation method and application thereof. The amino acid sequence of a heavy chain CDR region of the anti-human PD-1 mouse monoclonal antibody is selected from SEQ ID NO: 1-3 and/or SEQ ID NO: 7-9, and the amino acid sequence of a light chain CDR region of the anti-human PD-1 mouse monoclonal antibody is selected from SEQ ID NO: 4-6 and/or SEQ ID NO: 10-12. The invention belongs to the field of antibodies, and provides a protein sequence of an anti-human PD-1 mouse monoclonal antibody and recombinant expression of a stably transfected CHO cell strain for establishing the gene, the expressed and purified antibody can specifically recognize a human PD-1 antigen, and the expression yield of the antibody reaches the level of g/L and is far higher than that of an antibody prepared by traditional hybridoma cells. 本发明提供了抗人PD-1鼠单抗及其制备方法和应用，所述抗人PD-1鼠单抗重链CDR区氨基酸序列选自SEQ ID NO：1-3和/或SEQ ID NO：7-9，轻链CDR区氨基酸序列选自SEQ ID NO：4-6和/或SEQ ID NO：10-1