Evaluation method for release potential of greenhouse gas N2O of fluvial sediment

• Main Authors: NI JIE, ZHANG XUEJUN, WAN XIAOHONG, LANG HANG, ZHANG PANWEI, WU WENQIANG, HUANG AIPING, LI KUN, ZHAO XIAOHUI, GAO YUANYUAN, LIU YU
• Format: Patent
• Language: Chinese; English
• Published: 15.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a method for evaluating the release potential of greenhouse gas N2O of fluvial sediment, and belongs to the technical field of environment detection. The method comprises the step of determining the ratio of the gene abundance of nitrite reductase to the gene abundance of nitrous oxide reductase through fluorescent quantitative PCR (Polymerase Chain Reaction) so as to judge the release potential of greenhouse gas N2O in the river sediment. According to the evaluation method for the release potential of the fluvial sediment greenhouse gas N2O, disclosed by the invention, the release potential of the fluvial sediment greenhouse gas N2O with relatively low emission flux can be more accurately estimated. 本发明公开了一种河流沉积物温室气体N2O释放潜力的评价方法，属于环境检测技术领域。该方法为：通过荧光定量PCR测定亚硝酸盐还原酶基因丰度与氧化亚氮还原酶基因丰度的比值，以判定河流沉积物中温室气体N2O释放潜力。本发明公开的河流沉积物温室气体N2O释放潜力的评价方法，能够更加准确地估算排放通量相对较低的河流沉积物温室气体N2O释放潜力。