Low-temperature salt-tolerant beta-glucosidase and application thereof in conversion of ginsenoside Rb1

• Main Authors: ZHANG RUI, HUANG ZUNXI, ZHOU JUNPEI, GAO YANXIU, WU QIAN, CHENG YUNXIU, TANG XIANGHUA, ZHU HONGRONG
• Format: Patent
• Language: Chinese; English
• Published: 12.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a low-temperature salt-tolerant beta-glucosidase and application thereof in conversion of ginsenoside Rb1, and belongs to the technical field of genetic engineering, the enzyme is named as BglPcC2, and the amino acid sequence of the enzyme is shown as SEQ ID NO.1. The invention further discloses a preparation method of the low-temperature salt-tolerant beta-glucosidase. Through the mode, the beta-glucosidase BglPcC2 disclosed by the invention has low-temperature salt tolerance, the optimum temperature is 25 DEG C, and the residual enzyme activity is 65% or above after the beta-glucosidase BglPcC2 is treated at 10-35 DEG C for 1 hour; naCl or KCl with the mass concentration w/v of 3%-10% is adopted, so that the activity of the enzyme can be improved; the enzyme can efficiently and specifically convert almost all ginsenoside Rb1 into ginsenoside Rd within 1 h, the conversion rate reaches 97.81%, the conversion condition is mild, the product is single, later extraction is facilitated, and