Method for obtaining betulinol as adjuvant in vaccines against coronavirus SARS-COV-2

• Main Authors: KRASSILNIKOV IGOR VIKTOROVICH, IVANOV ALEXANDER VIKTOROVICH, ISAEV ARTUR ALEKSANDROVICH, FROLOVA MARIA EVGENEVNA, KUDRYAVTSEV ALEKSANDR VIKTOROVICH, VAKHRUSHEVA, ANA VLADIMIROVNA
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2023
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The present invention relates to biotechnology, and in particular to a method for the production of an adjuvant betulinol, which is suitable for the preparation of a vaccine against the coronavirus SARS-CoV-2. The invention relates to a method for producing a betulin, consisting in sterilising and filtering a tetrahydrofuran solution of betulin through a nylon membrane having a pore size of 0.22 [mu] m, reducing the tetrahydrofuran content by adding 25 times the volume of a sterile 0.01 M tris buffer (pH 9.0 +/-0.1), and subsequently homogenizing by ultrasound until a homogenized suspension is produced, spherical, amorphous, homogeneous particles suitable for binding to the SARS-CoV-2 viral protein are formed. The proposed technology makes it possible to produce betulinol with high sterility and immunogenicity, which improves the quality of vaccines against coronavirus. 本发明涉及生物技术，并且具体地涉及一种用于产生佐剂桦木醇的方法，该佐剂桦木醇适用于制备针对冠状病毒SARS-CoV-2的疫苗。该方法在于通过孔径为0.22μm的尼龙膜对桦木醇的四氢呋喃溶液进行灭菌过滤，通过添加25倍体积的无菌0.01M tris缓冲液(pH 9.0±0.1)来降低