Primer composition for detecting or assisting in detecting sugarcane mealybug, biological material and application thereof

• Main Authors: FANG ZHIPENG, HUANG PENGYING, LIAO FURONG, WU YUAN, CHEN JINGSONG, MA KEZHENG, LIN LINGLING
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer composition for detecting or assisting in detecting sugarcane mealybug, a biological material and application of the primer composition and the biological material. The technical problem to be solved by the invention is how to detect the mealybug sacchari with high sensitivity, strong specificity, good reproducibility and strong stability. In particular discloses a primer composition for identification or auxiliary identification of sugarcane mealybug, and is characterized in that the primer composition is composed of F1 and R1, the F1 is a single-stranded DNA molecule with a nucleotide sequence of SEQ ID No.1, and the R1 is a single-stranded DNA molecule with a nucleotide sequence of SEQ ID No.2. The primer group composition is used for carrying out in-vitro nucleic acid amplification on the to-be-detected mealybug, so that whether the to-be-detected mealybug is the sugarcane mealybug or not can be detected, and the primer group composition is high in sensitivity, specificity