Myc enhancer gene editing system and application thereof in preparation of product for treating estrogen receptor positive breast cancer

• Main Authors: LYU WANLIANG, XIE YING, BAO CHUNJIE, DUAN JIALUN
• Format: Patent
• Language: Chinese; English
• Published: 01.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a Myc enhancer gene editing system and application thereof in preparation of a product for treating estrogen receptor positive breast cancer. Experiments prove that the Myc enhancer gene editing system prepared by the invention can knock out an enhancer at the upstream of an estrogen receptor positive breast cancer gene Myc, knock out or damp the Myc enhancer, down-regulate Myc gene transcription and protein expression and inhibit estrogen receptor positive breast cancer cell proliferation on the premise of not damaging the Myc gene, so that the estrogen receptor positive breast cancer gene editing system can be used for preparing the estrogen receptor positive breast cancer gene. The apoptosis of the estrogen receptor positive breast cancer cells is induced, the cycle of the estrogen receptor positive breast cancer cells is retarded, and an efficient treatment effect on the estrogen receptor positive breast cancer is generated. The invention provides theoretical basis and experimental