Gulfweed germplasm preservation method

The invention relates to a gulfweed germplasm preservation method, and the principle is that the purpose of long-term preservation is achieved by utilizing the fact that the germplasm of a fertilized egg of gulfweed grows slowly and does not develop under specific conditions. The method comprises th...

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Bibliographic Details
Main Authors SANG SHILEI, QU YUANKAI, ZHAO ZHUANGMING, SUN LIWEI, XIE ENYI
Format Patent
LanguageChinese
English
Published 01.12.2023
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED
FISHING
FORESTRY
HORTICULTURE
HUMAN NECESSITIES
HUNTING
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
TRAPPING
WATERING
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Summary:The invention relates to a gulfweed germplasm preservation method, and the principle is that the purpose of long-term preservation is achieved by utilizing the fact that the germplasm of a fertilized egg of gulfweed grows slowly and does not develop under specific conditions. The method comprises the following steps: firstly, carrying out shade drying treatment on a sargassum encyneri reproductive tray, adding sterilized seawater for culture to obtain fertilized egg germplasm, respectively and sequentially culturing the fertilized egg germplasm in culture solutions with different formulas after the fertilized egg germplasm is obtained, then transferring the fertilized egg germplasm into liquid nitrogen for ultralow-temperature preservation, and taking out and reviving when seedling culture is needed. According to the method disclosed by the invention, in-vitro germplasm preservation is carried out on germplasm of the fertilized eggs of the sargassum enclosure, the germplasm preservation time can be prolonged
Bibliography:Application Number: CN202311044668