Method for selecting clone of cell expressing heterologous polypeptide

• Main Authors: GROSSKOPF THOMAS, WEZLER XAVIER
• Format: Patent
• Language: Chinese; English
• Published: 24.11.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Herein is reported a novel, high throughput, suitable method for characterizing and identifying and selecting recombinant cell clones expressing bispecific antibodies with high titers and low levels of antibody-related by-products. The method according to the invention uses the isolated binding to the corresponding target of the antibody and a contemporaneously bound binding signal for immunoassay-based screening of the recombinant cell clone culture supernatant. This data allows for the selection of highly productive cell clones with desired product profiles. 本文报道了一种用于表征和鉴定以及选择表达具有高滴度和低水平的抗体相关副产物的双特异性抗体的重组细胞克隆的新颖的、高通量的合适方法。根据本发明的方法使用针对所述抗体的相应靶标的分离结合以及同时结合的对重组细胞克隆培养上清液进行基于免疫测定的筛选的结合信号。该数据允许选择具有所需产物谱的高生产细胞克隆。