Means and methods for enhancing receptor-targeted gene transfer

• Main Authors: BRAUN ANDREAS, FRANKE ANDREAS, BUCHHOLZ CHRISTIAN
• Format: Patent
• Language: Chinese; English
• Published: 24.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of gene transfer. More specifically, the present invention relates to a method for enhancing the transfer of a receptor-targeted gene into a primitive T-cell as a host cell, the method comprising contacting a host cell comprised in a sample with a gene transfer vehicle in the presence of a tyrosine kinase inhibitor capable of inhibiting LCK tyrosine kinase in the host cell, the gene transfer vehicle comprises a targeting agent that specifically binds CD3 and a nucleic acid of interest to be transferred into the host cell, and the obtained host cell culture is cultured at a time and under conditions that allow receptor-targeted gene transfer. The present invention also relates to a method for preparing a medicament and a medicament comprising a gene transfer vehicle comprising a targeting agent that specifically binds CD3 and a nucleic acid of interest to be transferred into a primitive T-cell, and a tyrosine kinase inhibitor capable of inhibiting LCK tyrosine kinase in said