Typing application of Cashmere goat muscle growth and development functional genes MSTN and IGFBP-3

• Main Authors: HOU SIBING, LI JIAQI, WU YANZHI, LI SHUAITONG, WANG XIAOWEI, WANG ZEYING, LIU QINGKUN, LIU YINING, KONG LINGCHAO, SUN YINGGANG, LI SIYI, CHEN RUI, LI XI, PAN YUAN, ZHANG QIU, QIAO YANJUN
• Format: Patent
• Language: Chinese; English
• Published: 24.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of Cashmere goat muscle growth and development functional genes, in particular to typing application of Cashmere goat muscle growth and development functional genes MSTN and IGFBP-3. The typing application comprises the following steps: collecting a Cashmere goat blood sample: taking 1 ml of a blood sample from which DNA is extracted, putting the blood sample into a blood collection tube containing EDTA after blood collection, and storing at-20 DEG C; dNA is extracted, wherein an EZ-10 rotating column blood genome DNA purification kit is used for DNA extraction, and preservation is conducted at-20 DEG C; body size performance data: measuring body size measurement data according to a livestock breeding experiment method. According to the invention, SNP is utilized to carry out genetic typing on the muscle growth and development functional genes MSTN and IGFBP-3 of the cashmere goat, SNP loci are found and are subjected to genetic diversity analysis, and genotype and