Traditional Chinese medicine compound fingerprint spectrum detection method established based on continuous extraction strategy

• Main Authors: GAO YAN, ZHAO BONIAN, DU RUIQING, LIU LU, YU ZIXIANG
• Format: Patent
• Language: Chinese; English
• Published: 21.11.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of traditional Chinese medicine compound component analysis and detection, and particularly relates to a traditional Chinese medicine compound fingerprint spectrum detection method established based on a continuous extraction strategy. The method is realized through the following steps: firstly, continuously extracting a test article Jingzhi granules by adopting extraction solvents with different polarities, and respectively preparing test solutions of different polar parts; preparing an internal standard solution; and carrying out fingerprint detection and quantitative characterization on the test solution by adopting ultra-high performance liquid chromatography combined with mass spectrometry, and carrying out attribution analysis on each component. According to the method, different combined solvent extraction methods are compared through qualitative and quantitative analysis, the optimal continuous extraction strategy is determined, the extraction method is opt