Process method for extracting and preparing urechis unicinctus heme

• Main Authors: JIAO XUDONG, DONG HAOMIAO, HUANG DONG, XU SHUCHAO
• Format: Patent
• Language: Chinese; English
• Published: 21.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of comprehensive utilization of biological resources, and particularly relates to a process method for extracting and preparing urechis unicinctus heme. The method specifically comprises the following steps: filtering fresh urechis unicinctus body cavity fluid with filter cloth, centrifuging, and discarding supernatant; adding 2-3 times of cleaning liquid to wash the red blood cells, centrifuging for 5-30 minutes again, collecting the red blood cells, and repeating for 1-3 times to obtain the required red blood cells; centrifuging for 10-30 minutes under a certain condition, discarding the precipitate, collecting the supernate, and freeze-drying to obtain the hemoglobin freeze-dried powder; the method comprises the following steps: dissolving hemoglobin freeze-dried powder according to a substrate concentration of 20 g/L, adding protease with a mass of 2000-10000 U/g, adjusting the temperature of the solution to 35-55 DEG C, adjusting the pH value of the solution to 5-10, ca