Construction method of osteosarcoma induced pluripotent stem cell platform

The invention discloses a construction method of an osteosarcoma induced pluripotent stem cell platform, which comprises the following steps: preparing induced pluripotent stem cells of osteosarcoma, transferring pluripotent factors OCT4, SOX2, CMYC and KLF4 into osteosarcoma cells, and carrying out...

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Bibliographic Details
Main Authors PAN SIQIONG, CHEN MIAOYU, TAN LINGFENG, KORETOSHI, QIAO SHUYE
Format Patent
LanguageChinese
English
Published 21.11.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a construction method of an osteosarcoma induced pluripotent stem cell platform, which comprises the following steps: preparing induced pluripotent stem cells of osteosarcoma, transferring pluripotent factors OCT4, SOX2, CMYC and KLF4 into osteosarcoma cells, and carrying out induced culture to obtain cloned induced pluripotent stem cells; in the process of re-differentiating induced pluripotent stem cells into osteosarcoma cells, through systematic comparison of wild type and disease osteoblasts, potential pathological mechanisms related to osteosarcoma development can be clarified, the pathogenesis of osteosarcoma which is not represented in the prior art can be clarified, and potential treatment strategies for treating osteosarcoma patients can be developed. The platform is applied to construction of other disease models, research on pathogenesis of diseases and development of treatment schemes. 本发明公开了骨肉瘤诱导多能干细胞平台的构建方法，通过制备骨肉瘤的诱导多能干细胞，将多能性因子OCT4、SOX2、CMYC、KLF4转入骨肉瘤细胞内，经过诱导培养，获得类克隆的诱
Bibliography:Application Number: CN202311096288