InDel molecular marker primer for identifying white gourd peel color and application of InDel molecular marker primer
The invention discloses an InDel molecular marker primer for identifying the color of white gourd peel. The InDel molecular marker primer is one or two of a PS830 primer and a PS208 primer; wherein the nucleotide sequence of an upstream primer of the PS830 primer is as shown in SEQ ID NO.1, and the...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
17.11.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses an InDel molecular marker primer for identifying the color of white gourd peel. The InDel molecular marker primer is one or two of a PS830 primer and a PS208 primer; wherein the nucleotide sequence of an upstream primer of the PS830 primer is as shown in SEQ ID NO.1, and the nucleotide sequence of a downstream primer of the PS830 primer is as shown in SEQ ID NO.2; the nucleotide sequence of an upstream primer of the PS208 primer is as shown in SEQ ID NO.3, and the nucleotide sequence of a downstream primer of the PS208 primer is as shown in SEQ ID NO.4. The method comprises the following steps: comparing nucleotide sequences of chromosomes 5 of white gourd varieties with different skin colors, designing an InDel marker covering the chromosome 5 of a white gourd genome, and carrying out polymorphism screening on the developed InDel marker by virtue of a white gourd parent and a hybridization F1 pair. And judging that the PS830 and the PS208 are codominant markers. And designing an InDel |
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Bibliography: | Application Number: CN202311004142 |