METHOD FOR DETECTING ASTER-B AS PERIPHERAL NERVE REMYELINING THERAPEUTIC TARGET

• Main Authors: SU WENFENG, CHEN GANG, GU YUN, XING LINGYAN, WEI ZHONGYA, ZHAO YAYU
• Format: Patent
• Language: Chinese; English
• Published: 17.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a method for detecting Aster-B as a peripheral nerve remyelination treatment target spot, and the scheme specifically comprises the following steps: firstly culturing and purifying Schwann cells, then culturing and purifying DRG neurons, then co-culturing the DRG neurons and the Schwann cells, then separating myelin sheaths through laser microdissection (LCM) to form the Schwann cells, and finally detecting the Aster-B as the peripheral nerve remyelination treatment target spot. Western blot (protein coloring), cell RNA (Ribonucleic Acid) extraction and reverse transcription and fluorescent quantitative PCR (Polymerase Chain Reaction) are realized by establishing a Schwann cell differentiation model and a sciatic nerve pinch injury model. Through sciatic nerve microinjection and a sciatic nerve pinch injury model, the mouse peripheral nerve axon myelin sheath form is observed by using a transmission electron microscope. By adopting the technical scheme, the effect of the Aster-B on ref