Genome protozoa pollutant detection method based on DNA bar code technology

• Main Authors: XIE JIAZHENG, ZHANG YI
• Format: Patent
• Language: Chinese; English
• Published: 14.11.2023
• Subjects: INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; PHYSICS

The invention belongs to the field of genome pollution sequence identification and pollutant species analysis and search, and particularly relates to a genome protozoa pollutant detection method based on a DNA bar code technology, which comprises the following steps: acquiring DNA sequence data to be detected; screening the DNA sequence data by adopting three genomic contamination screening strategies, and performing sequence comparison on the screened DNA sequence data and a Genbank database sub-library nt database to select an optimal comparison result; wherein the three genome pollution screening strategies are contigs length screening, comparison with a protist DNA sequence and non-protist DNA sequence data respectively; according to the method, DNA sequence data is screened through three genome pollution screening strategies, so that the pollution identification false positive is reduced, and the detection accuracy is improved. 本发明属于基因组污染序列的鉴定及污染物种分析查找领域，具体涉及一种基于DNA条形码技术的基因组原生动物污染物检测方法，包括：获取待检测的DNA序列数据；采