Tissue slice image and DAPI dyeing image registration method

• Main Authors: WANG TAIPING, ZHANG MINFEI
• Format: Patent
• Language: Chinese; English
• Published: 14.11.2023
• Subjects: CALCULATING; COMPUTING; COUNTING; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL; PHYSICS

The invention relates to the technical field of pathological image processing, and provides a tissue slice image and DAPI dyed image registration method, which comprises the following steps: carrying out gray processing on a tissue slice image and a DAPI dyed image, and amplifying according to the same working multiplying power; acquiring a feature point image of the tissue slice and DAPI dyeing; searching an optimal scaling ratio is optimal, a rotation angle ia, a horizontal displacement dx, a vertical displacement dy and an average point (xm, ym) of feature point images of the tissue slice and DAPI dyeing; generating a plurality of sub-frames by using the average points (xm, ym), and enabling the tissue slice feature points contained in the sub-frames to exceed 200; for each sub-frame, processing DAPI dyeing feature points to generate a transformation matrix tf2 by using an optimal scaling is optimal and a rotation angle ia under the condition that (xm, ym) is taken as a rotation center; and generating a fi